Human Tryptase Alpha/Beta-1 (TPSAB1) ELISA Kit

Catalog No:        abx153400

Size:                    96T

Range:                7.8 pg/ml - 500 pg/ml

Sensitivity:          < 2.8 pg/ml

Storage:              Store the 96-well plate, Standards, and Detection Reagent(s) at -20°C, and the rest of the kit components at 4°C.

Application:      The quantitative detection of TPSAB1 in Human serum, plasma and other biological fluids.Principle of the Assay: This                                kit is based on sandwich enzyme-linked immuno-sorbent assay technology. An antibody is pre-coated onto a

                          96-well plate. Standards, test samples, and biotin-conjugated reagent are added to the wells and incubated. The                                      HRP-conjugated reagent is then added, and the whole plate is incubated. Unbound conjugates are removed using                                  wash buffer at each stage. TMB substrate is used to quantify the HRP enzymatic reaction. After TMB substrate is                                      added, only wells that contain sufficient TPSAB1 will produce a bluecoloured product, which then changes to yellow                                after adding the acidic stop solution. The intensity of the yellow colour is proportional to the TPSAB1 amount bound                                on the plate. The Optical Density (OD) is measured spectrophotometrically at 450 nm in a microplate reader, from                                    which the concentration of TPSAB1 can be calculated.

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